We showed that Open-CSAM was more accurate to measure CSA in regenerating and dystrophic muscles as compared with SMASH, MyoVision, and MuscleJ softwares and that the inter-experimenter variability was negligible.
WhatsApp: +86 18221755073Textile Waste Fiber Regeneration via a Green Chemistry Approach: A Molecular Strategy for Sustainable Fashion Adv Mater. 2021 Dec;33(48): e2105174. ... However, waste textiles and fibers are still mainly disposed of in landfills or by incineration after their service life and thereby pollute the natural environment, as there is still no ...
WhatsApp: +86 18221755073In this study, we propose an automated image analysis system for quantitative characterization of muscle fiber type composition.
WhatsApp: +86 18221755073This is also an important parameter for characterization of regenerating fibers as it is a readout of regeneration efficiency. Running MuscleJ on muscle sections co-labeled with laminin and DAPI returns the number of regenerated fibers and the number of nuclei in each fiber. ... Smith LR, Barton ER. SMASH - semi-automatic muscle analysis using ...
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WhatsApp: +86 18221755073The dissolution of silk is a critical step in producing regenerated silk fibroin (RSF) fibers with superior performance [5], [6], [7].Native silk has been dissolved in H 3 PO 4 [8], H 3 PO 4 –formic acid (FA) [9], LiBr (self-dialysis) [10] or ionic liquids [11] for direct spinning of fibers; although these processes are simple the resulting fibers perform poorly.
WhatsApp: +86 18221755073Here, we have developed a novel fully-automated image segmentation method based on neutrosophic set algorithms to analyse whole skeletal muscle cross sections in …
WhatsApp: +86 18221755073Background: Optical fiber sensors have been used to detect glucose owing to advantages such as low cost, small size, and ease of operation etc. phenylboronic acid is one of the commonly used receptors for glucose detection, however phenylboronic acid based regenerative optical fiber sensors are commonly cumulative regeneration, renewable …
WhatsApp: +86 18221755073Here, we have developed a novel fully-automated image segmentation method based on neutrosophic set algorithms to analyse whole skeletal muscle cross sections in exercise-induced regenerating myofibers, referred as MyoView, designed to obtain accurate fiber size and distribution measurements.
WhatsApp: +86 18221755073Histological parameters including fiber distribution, fiber type, centrally nucleated fibers, and capillary density are all frequently quantified measures of skeletal muscle. These parameters …
WhatsApp: +86 18221755073In this study, we propose an automated image analysis system for quantitative characterization of muscle fiber type composition.
WhatsApp: +86 18221755073Regeneration of the muscle fibers is apparent as a shift of myonuclei from the periphery of the fibers toward the center [1]. Fibrosis also becomes evident as a buildup of extracellular matrix (ECM) within the muscle's interstitial ... SMASH 2.0: …
WhatsApp: +86 18221755073The main parameter studied to assess muscle regeneration efficacy is the cross-sectional area (CSA) of the myofibers as myofiber size correlates with muscle force. ... when performed manually. This is why programs were developed to completely automate the analysis of the CSA, such as SMASH, MyoVision, or MuscleJ softwares. ... Muscle Fibers ...
WhatsApp: +86 18221755073neurostimulation on nerve regeneration between the proximal and distal nerve stumps in diabetic rats.42–45 Therefore, we hypothe-sized that neurostimulation for pain treatment at the level of the DRG might also lead to intraepidermal nerve fiber regeneration in SFN. The primary aim of this study was to assess the impact of
WhatsApp: +86 18221755073We showed that Open-CSAM was more accurate to measure CSA in regenerating and dystrophic muscles as compared with SMASH, MyoVision, and MuscleJ softwares and that the inter-experimenter variability was negligible.
WhatsApp: +86 18221755073All animal experiments were approved by the University of Pennsylvania Institutional Animal Care and Use Committee. C57Bl/6 mice were used as wild-type controls and mdxmice were used as a dystrophic model. Both animal groups were analyzed at 1 year of age.
WhatsApp: +86 18221755073Initial segmentation and fiber filter functions segment the image and remove non-fiber elements based on user-defined parameters to create a fiber mask. Establishing …
WhatsApp: +86 18221755073In particular, centrally nucleated fibers are often used as a marker for muscle regeneration. While fully mature fibers have peripheral nuclei, newly regenerated fibers have central nuclei [15].
WhatsApp: +86 18221755073Initial segmentation and fiber filter functions segment the image and remove non-fiber elements based on user-defined parameters to create a fiber mask. Establishing parameters set by the user,...
WhatsApp: +86 18221755073In particular, centrally nucleated fibers are often used as a marker for muscle regeneration. While fully mature fibers have peripheral nuclei, newly regenerated fibers have central nuclei [15]. In many muscular dystrophies, which are …
WhatsApp: +86 18221755073We successfully applied our segmentation pipeline to uncover myofiber regeneration differences between two different muscle injury models, cardiotoxin and glycerol. …
WhatsApp: +86 18221755073In our study, we investigated whether the deletion of miR-378a (miR-378) affects muscle regeneration. We subjected 6-week-old wild-type (WT) and miR-378 knockout (miR-378–/–) animals to the...
WhatsApp: +86 18221755073Histological parameters including fiber distribution, fiber type, centrally nucleated fibers, and capillary density are all frequently quantified measures of skeletal muscle. These parameters reflect functional properties of muscle and undergo adaptation in …
WhatsApp: +86 18221755073However, as the mdx muscle is undergoing continual cycles of degeneration and regeneration the variability in fiber CSA is much greater than wild-type muscle [2, 31]. ... Dark gray areas are interstitial in SMASH and fibers using legacy methods while white areas are interstitial in both masks. Figure demonstrates the larger fiber area obtained ...
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WhatsApp: +86 18221755073Download Table | Data comparison from legacy methods to SMASH from publication: SMASH - semi-automatic muscle analysis using segmentation of histology: A MATLAB application | Histological ...
WhatsApp: +86 18221755073Here, we have developed a novel fully-automated image segmentation method based on neutrosophic set algorithms to analyse whole skeletal muscle cross sections in exercise-induced regenerating...
WhatsApp: +86 18221755073Figure demonstrates the larger fiber area obtained with SMASH compared to the legacy method of using a simple threshold for fiber area. from publication: SMASH - semi-automatic muscle...
WhatsApp: +86 18221755073the regeneration of natural fibers usually employ concentrated acidic or alkaline solutions, which destroy the polymer struc-tures of fibers, rendering them unusable as fibers in textiles and fashion. Moreover, these chemical processes unavoidably introduce hazardous substances and have been strictly pro-hibited in some countries.
WhatsApp: +86 18221755073In our study, we investigated whether the deletion of miR-378a (miR-378) affects muscle regeneration. We subjected 6-week-old wild-type (WT) and miR-378 knockout (miR-378–/–) animals to the...
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